Enzyme immunoassay for detecting Brucella antibodies in cow's milk

Enzyme immunoassay for detecting Brucella antibodies in cow's milk.

An enzyme immunoassay (EIA) was developed for detecting Brucella antibodies in milk of cows infected with Brucella abortus. The enzyme immunoassay using heat-killed cells of B. abortus strain 19 was of comparable sensitivity to the Brucella ring test in detecting antibodies in milk of a reference positive control cow experimentally infected with B. abortus strain 2308 and in milk of 16 naturall...

Enzyme-immunoassay for determination of progesterone in milk from cows.

An enzyme-immunoassay using horseradish peroxidase as label is described. The assay has a sensitivity of 0.1-20 pmol, i.e. 0.03-0.63 ng and intra- and inter-assay coefficients of variation of less than 10% when used for the determination of progesterone in samples of milk from cows. Comparison with a radio-immunoassay based on tritiated tracer showed excellent correlation (r = 0.98) for milk sa...

Pretreatment-free lateral flow enzyme immunoassay for progesterone detection in whole cows' milk.

New rapid method of lateral flow enzyme immunoassay (LFEIA) for progesterone detection in whole cows' milk was developed. The test system utilized horseradish peroxidase as a label along with the substrate solution containing 3,3',5,5'-tetramethylbenzidine and dextran sulfate to obtain an insoluble blue colored product of the enzyme reaction on a surface of analytical membrane (test and control...

Indirect enzyme-linked immunosorbent assay for detection of Brucella melitensis-specific antibodies in goat milk.

Brucella melitensis is the cause of brucellosis in sheep and goats, which often results in abortion. Few cases of B. melitensis infection in goats have occurred in the United States over the last 25 years. However, vigilance must be maintained, as it is for the bovine milk industry, to ensure that brucellosis is not introduced into the U.S. goat population. The objective of this study was to de...

lsolation of Brucella Organisms from the Milk of Seronegative Cows